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ELISA- A DIAGNOSTIC TOOL FOR MONITORING OF NIPAH VIRUS IN PIGS, DOGS, CATS AND HORSE IN THE VETERINARY RESEARCH INSTITUTE (VRI), IPOH, MALAYSIA (2011)

The outbreak of Nipah viral encephalitis in 1999 caused fatalities in both human and pigs. The outbreak caused mass culling of nearly one ,million pigs to controlthe outbreak. A national surveillance program was scheduled by the Department OF Veterinary Services (DVS) to screen all pigs farms in Malaysia and animals to be exported against Nipah virus antibodies. For speed and safety, an indirect ELISA was developed to screen those animals. ELISA serology can be conducted safely and quickly without access to PC4 facilities, and can be a most useful diagnostic tool. The ELISA plates used in the test were coated with inactivated Nipah virus antigen. The specificity and sensitivity of the ELISA was calculated to be 97.5% and 80% respectively comparable to the serum neutralization test (SNT). Total of 4751 serum samples have been tested to study the efficacy of the in-house kit.

Faizul Fikri, My., Che Ku Mardianty,S. Letchumi, Dr Ramlan.

Veterinary Research Institute, 59 Jalan Sultan Azlan Shah, 31400, Ipoh, Perak, Malaysia

Corresponding Author: faizul@jphvri.gov.my

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Faizul Fikri, My., Che Ku Mardianty,S. Letchumi, Dr Ramlan. ELISA- A DIAGNOSTIC TOOL FOR MONITORING OF NIPAH VIRUS IN PIGS, DOGS, CATS AND HORSE IN THE VETERINARY RESEARCH INSTITUTE (VRI), IPOH, MALAYSIA (2011). (23rd VAM CONGRESS 2011)


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