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ANALYSIS OF CYROMAZINE IN POULTRY FEED AND MUSCLES USING LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY

Cyromazine is a registered pesticide for controlling flies  in poultry-caged layers.  The compound is added in feed and passed through the chicken, leaving

a residuin the manure that controls the growth of the fly larvae developing  the area

Concentration of cyromazine were determined in poultry feed and muscle using a liquid chromatography-mass spectrometry (LC-MS) coupled with electrospray ionisation. The procedure was optimised for simultaneous identification and quantitation of cyromazine and its metabolite. melamine in feed and poultry muscles. The compounds were extracted using QuEChERS  method followed by dSPE C18 clean-up. LC separation was achieved usi ng Atlantis HI LIC silica column. Selective ion reaction (SIR) was used for selective dett:ction of cyromazine and melamine. The calibration curves were linear in the 0' 10, 25 , 50, 75 and 100 µglkg range, with typical (R2) values higher than 0.99  for cyromazine and 0.98 for melamine. The LOD and LOQ were lower than the MRL  for cyromazine which is 50 µg/kg. This method was successfully applied to real samples for the analysis j of cyromazine in poultry feed and muscles.

 

Keywords: cyromazine, melamine, poultry, tissue, feed, LC-MS

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About this Article Paper

Faridah I. And Suhaimi D (2011). Analysis Of Cyromazine In Poultry Feed And Muscles Using Liquid Chromatography–Mass Spectrometry. 23rd Veterinary Association Malaysia Congress (VAM) 2011 Hotel Tower Regency Ipoh, Perak 23rd-24th September 2011.


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