All Categories


Pages

First detection of Bovine Viral Diarrhea Virus in swine from Malaysia

Bovine viral diarrhea virus (BVDV) naturally infects cattle and results in mucosal disease, acute infections of respiratory system and gastrointestinal tracts and reproductive disorders. However, since 1973, the host range of BVDV has been found to extend  to swine, sometimes causing symptoms mimicking those of Classical Swine Fever Virus (CSFV). We report the first detection of BVDV in swine from Sabah, Malaysia. A pooled swine tonsils were received by Veterinary Research Institute (VRI) on June 2017 for CSFV surveillance and detection was carried out using reverse transcriptase polymerase chain reaction (RT-PCR). A 288 bp amplicon from the 5′ untranslated region (5′ UTR) of the genome was successfully amplified using primers 324 and 326. Purified amplicon is further sent for sequencing for virus confirmation. Phylogenetic analysis of the sequencing result was done by Neighbour-Joining method, with CSFV as an outgroup. Analysis comprised of additional sequences of BVDV-1 and BVDV-2 genotypes taken from GenBank and indicated that this virus clustered with BVDV-2. The virus sequence was deposited in GenBank with accession number MH814636. This finding may have important implications for the epidemiology, diagnosis and control of BVDV infection in the country.   

Keywords: Bovine viral diarrhea virus (BVDV), Classical Swine Fever Virus (CSFV), reverse transcriptase polymerase chain reaction (RT-PCR), Phylogenetic analysis 

download


More Info

Upload By : Debbra Marcel

About this Article Paper

Nurain Izzati Saidi. First detection of Bovine Viral Diarrhea Virus in swine from Malaysia. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).

 

1Nurain Izzati Saidi, 2Norlina Daiyan, 2Roshaslinda Dahlan, 2Roslina Hassan   1Veterinary Public Health Laboratory, Department of Veterinary Services. Bandar Baru Salak Tinggi, 43900 Sepang, Selangor;  2Veterinary Research Institute, Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia  Corresponding author: nurain@dvs.gov.my  


Comments


No comments yet! Be the first:

Your Response



Most Viewed - All Categories