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MOLECULAR DETECTION OF INFECTIOUS BURSAL DISEASE FROM A SAMPLE SUBMITTED TO VRI.

A sample suspected of Infectious Bursal Disease (IBD) was positive by AGPT via virus isolation as well as molecular detection. In molecular detection, RT-PCR was able to amplify 474 bp of the variable regions of VP2 gene from the isolate. Digestion with three restriction enzymes Hinf I, Ssp I and Taq I showed patterns compatible with the vaccine strain of IBD. The sequence analysis of the variable region of VP2 gene showed that the isolate was closely related to the IBD vaccine strains.

A.S. Syamsiah, M.Y. Faizul F., G.H. Ong, B.L. Leow, S. M. Redzuwan, M.A. Basirah, M. Hasrul A.H., Z. Maizatul, B. Zunaida, M.R. Jihan and M. Ramlan

Veterinary Research Institute, Department of Veterinary Services. 59 Jalan Sultan Azlan Shah, 31400 Ipoh, Perak.

Corresponding author: syamsiah@dvs.gov.my

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A.S. Syamsiah, M.Y. Faizul F., G.H. Ong, B.L. Leow, S. M. Redzuwan, M.A. Basirah, M. Hasrul A.H., Z. Maizatul, B. Zunaida, M.R. Jihan and M. Ramlan. Molecular Detection Of Infectious Bursal Disease From A Sample Submitted To VRI. Proceeding of 25th VAM Congress 2013, Lotus Desaru Beach Resort, Johor, Malaysia. (pp 62)


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