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Protective efficacy of recombinant Newcastle Disease subunit vaccine against challenge with Malaysian genotype VII Newcastle Disease Virus

Recombinant baculoviruses containing the hemagglutinin-neuraminidase (rHN) glycoprotein coding region of Malaysian Genotype VII (gVII) Newcastle disease virus (NDV) was constructed in an attempt to develop an effective subunit vaccine against Malaysian gVII NDV. Protective effect of the subunit vaccine was determined by accessing haemagglutination inhibition (HI) antibody titer, clinical signs, mortality and virus shedding after challenge with Malaysian gVII NDV 12234/2010 strain (MAY-gVII-NDV-12234/10). SPF chickens were divided into five groups, and each group was vaccinated once subcutaneously with the Seppic-adjuvanted antigen of different rHN concentrations (104 cell/ dose, 105 cell/dose, 106 cell/dose, 107 cell/dose and uninfected cell). The immunized chickens were then challenged with 2x 105 EID50 per 0.1ml of gVII NDV (MAY-gVII-NDV-12234) at 14 days post vaccination (dpv) and closely monitored for mortality and clinical signs. The gVII recombinant ND HN (rNDHN) glycoproteins expressed in Sf21 cells exhibited good protection against clinical signs and mortality, showing a significant difference (p<0.05) in HI antibody titer of the group receiving 107 cell/dose in comparison to groups receiving 104, 105 and 106 per dose at 14 dpv with mean HI titers (Log2 ± SD) 4.1 ± 0.7 , < 1, 1.1 ± 0.7 and  3.3 ± 0.7 respectively. The vaccine provides 100% (15/15) protection against clinical signs and mortality in both groups with 107 cell/dose and 106 cell/dose and suppresses virus shedding by 60%. The findings suggest that the rNDHN glycoprotein derived from local gVII NDV can be a subunit vaccine candidate for chickens in a future ND eradication and control programme.    

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About this Article Paper

Suriani Mohd Noor. Protective efficacy of recombinant Newcastle Disease subunit vaccine against challenge with Malaysian genotype VII Newcastle Disease Virus. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of Rapid Oral Presentation).

 

1Suriani Mohd Noor, 2Liew Mervyn Wing On, 2Lye Ping Ying, 1Niny Fariza Junoh, 1Abdul Jabir Jaafar, 1Syamsiah Aini Shohaimi, 1Faizul Fikri Mohd Yusop, 1Chin Saw Wang, 1Goon Swee Cheong, 1Jamaliah Hamid  

1Veterinary Research Institute, Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia 

2Institute for Research in Molecular Medicine (INFORMM), University Sains Malaysia,  11800, Minden, Pulau Pinang, Malaysia  

Corresponding author: surianivri@gmail.com 


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