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Evaluation of diagnostic sensitivity and specificity of Melioidosis Complement Fixation Test in sheep and goat using 2124/01 Burkholderia pseudomallei antigen

 In Malaysia, Melioidosis in animals has been reported in local goats, sheep, cattle, pigs, deer, monkeys, horses, cats, dogs, and rabbits since 1921. It was also reported among orang utans at the Sepilok Rehabilitation Centre in Sandakan, Sabah in 1965 and 1968, where Burkholderia pseudomallei was successfully isolated. Many reports also suggested a wide distribution of B. pseudomallei in the environment in Malaysia. The serological test that is currently available in Veterinary Research Institute Ipoh is the complement fixation test (CFT) for the detection of antibodies in animals, but the diagnostic sensitivity (DSe) and diagnostic specificity (DSp) of the assay have not been previously established for use in sheep and goats in Malaysia. Therefore, an attempt to produce B. pseudomallei 2124/01 (local isolate) antigen for complement fixation test has been initiated in order to provide the information needed. Analysis of both DSe and DSp were conducted with reference to the ‘OIE Manual of Terrestrial Animals Chapter 1.1.1. Principles and Methods of Validation of Diagnostic Assays for Infectious Diseases’ at 95% confidence interval and 5% error for estimation of DSe and DSp. As a results, the DSe of the CFT assay using local isolates antigen is 97.8% while the DSp is 100%. In conclusion, the CFT assay using B. pseudomallei 2124/01 antigen is fit to be used as screening and confirmation of antibodies in sheep and goats in Malaysia. 

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Nurul Fatiha Ahmad Shuhaimy. Evaluation of diagnostic sensitivity and specificity of Melioidosis Complement Fixation Test in sheep and goat using 2124/01 Burkholderia pseudomallei antigen. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of Rapid Oral Presentation).

 

*Nurul Fatiha Ahmad Shuhaimy, Rozza Nadiah Rosli, Azizah Darus  Veterinary Research Institute Ipoh  *Corresponding Author: nurulfatiha@dvs.gov.my 


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