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Histopathological and virus tissue distribution of Avian Reovirus Cluster IV in Malaysia

 Avian Reovirus (ARV) has been known to cause a wide range of disease presentations affecting the avian species. This study was performed to compare the microscopic lesions induced by ARVs histologically in six different tissues such as tendon, liver, lung, heart, spleen, and kidney. Tissues samples from 63 weeks old of an infected broiler breeder chickens were collected at four different processing plants in Johor. The tissues were fixed in 10% neutral buffered formalin. After that, the tissues were trimmed, embedded in paraffin, sectioned at 3-5 µm, mounted, stained with hematoxylin and eosin (H&E) and microscopically examined under light microscope. Lesions were scored as no lesions (-), minimal (+/-), mild (+), moderate (++) and severe (+++). Histopathological examination of the  tissues revealed different degrees of edema, suppurative infiltration, perivascular cuffing, inflammation, necrosis and many other types of lesions. The tendon tissues were severely damaged when compared to the mildly affected liver, heart, spleen, lung, and kidney. These observed findings support the detection of a pathogenic strain of ARVs Genotype Cluster IV by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) that was confirmed by DNA sequencing. Hence it also tallies with the farmer’s complaint during a field investigation in mid-April 2018. In conclusion, this study verifies that ARV had replicated and spread in all the tissues samples that were taken in varying degrees. The severity of the lesions proves the higher replication of ARV in the tissues.    

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About this Article Paper

Norazura A. Hamid. Histopathological and virus tissue distribution of Avian Reovirus Cluster IV in Malaysia. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of Rapid Oral Presentation).


 

Norazura A. Hamid, Veterinary Research Institute, Corresponding Author: zurasmarties@gmail.com 


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