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A comparison of the straw technique and conventional technique for sample collection for Rabies FAT

Rabies is caused by Lyssavirus of the Rhabdovirus family. Rabies was recently reported in 2017 in Sarawak and 2018 in Perlis. The straw technique requires an ordinary drinking straw and skill for the brain sampling. On the other hand, the conventional technique for brain removal requires separation of the head from the spine by cutting through the cervical spine at the atlanto-occipital joint and removal of the whole brain. The straw technique is suitable for transportation of fresh brain sample from an isolated area where samples could be transported in glycerol instead of the whole head on the ice. The conventional technique comes in handy when the distance of the laboratory facility is nearer to the sampling site. Besides that, appropriate equipments, skills, and ability of the operators are dependable in both techniques. The conventional technique more laborious as physical strength is needed. The straw technique is less laborious as it only requires a sharp knife, straw, and skill. The size of the head is important in determining the type of brain removal procedure. The results produced by Fluorescent Antibody Test (FAT) was similar in both techniques. There are many pro and cons when using the straw technique compared to the conventional technique. Therefore, the straw technique is safe and practical to perform as compared to the conventional with some limitation included.    

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About this Article Paper

Norazura A. Hamid. A comparison of the straw technique and conventional technique for sample collection for Rabies FAT. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of Rapid Oral Presentation).


 

Norazura A. Hamid, Veterinary Research Institute, Corresponding Author: zurasmarties@gmail.com 


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