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Evaluation of whole blood test kit for Salmonella screening for poultry

Salmonellosis is an important disease in poultry. Flock screening using rapid salmonella stained antigen is recommended by OIE for rapid diagnosis. A study was conducted to evaluate the performance of Salmonella stained antigen produced by VRI (SAPEN) and  the commercial antigen. Twenty minimum disease-free chicken of five weeks old were divided into four group, and three of the group were inoculated with three Salmonella serovar; Salmonella Pullorum, Salmonella Enteritidis and Salmonella Corvallis respectively to induce the hyperimmune sera production. The chicken blood were tested with both antigens, one to two weeks before the immunization. Performance of both antigens were evaluated based on the detection of seropositive chicken and speed of the reaction. Generally, all the immunised chicken were positive for Salmonella using both stained antigen while the non-treatment chicken was negative. Chicken inoculated with S. Enteritidis were 100% tested positive using both antigens throughout the experiment. However, the chicken inoculated with S.Pullorum showed 40% to 80% positive reaction within 14 to 48 days p-i when tested with SAPEN compared to 100% positive of the commercial antigen. This study suggested that both antigens perform equally well for S. Enteritidis antibody detection based on seropositive birds while the commercial antigen performs better than SAPEN in the detection of S. Pullorum and S. Enteritidis antibody based on the reaction time. Thus, it is crucial to the established improved version of local stained antigen for effective diagnosis of the disease in the farm.    

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Dahlia Harun. Evaluation of whole blood test kit for Salmonella screening for poultry. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of Rapid Oral Presentation).

 


*Dahlia Harun, Muhamad Marwan Ibrahim, Zarrahimah Zakaria, Nor Liyana Mohamas Razak,  Zurin Azlin Mohamad Jinin, Sohayati Abdul Rahman
Veterinary Research Institute
*Corresponding Author: dahlia@dvs.gov.my 

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