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EVALUATION OF MICROSATELLITE MARKERS FOR PARENTAGE VERIFICATION IN CATTLE

Abstract: Traditionally, parentage verification in cattle has been performed using blood typing. Parentage verification is very important, since it is the basis for breeding schemes and becomes even more critical with the widespread use of artificial insemination and embryo transfer techniques. Recently, a new methodology employing DNA markers is being used for parentage control. In order to achieve this goal, information regarding the amount of genetic variation of the microsatellites must be obtained. In the present study, we assessed the polymorphism of 13 microsatellites in regularly reared cattle breed in Malaysia using conventional PCR and genotyping technique. A total of 90 animals belonging to 3 cattle breeds (Kedah-Kelantan, Brahman, Mafriwal) and reared in government Livestock Breeding Center were sampled. Heterozygosity, polymorphism information content, and exclusion probability of the microsatellite were calculated. Considering the 13 microsatellites, exclusion probabilities were as follows: Kedah-Kelantan, 0.5910; Brahman, 0.4506; Mafriwal, 0.5790. All of the thirteen genetic markers in the 3 cattle breeds analyzed, except BM 1824 and ETH 10, were highly informative and their exclusion probabilities demonstrate their usefulness for cattle parentage verification in Malaysia.

 

Keywords: Parentage verification, microsatellite, cattle, Cervus 3.0

 

 

Mohd Hafiz A.R.1, Zawawi I.2, Mohd Hafizal A.1 Salleh S. I.1 Ernie-Muneerah M. A.1

 

1Institut Biodiversiti Veterinar Kebangsaan, 27000 Jerantut, Pahang

E-mail:  hafiz@dvs.gov.my

2Pusat Ternakan Haiwan Tersat, 21700 Kuala Berang, Terengganu

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Mohd Hafiz A.R.1, Zawawi I.2, Mohd Hafizal A.1 Salleh S. I.1 Ernie-Muneerah M. A.1

 


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