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INACTIVATION/DELETION OF PLD GENE FROM Corynebacterium pseudotuberculosis

Caseous lymphadenitis is a disease caused by C. pseudotuberculosis which brings significant economic losses to small ruminant producers worldwide. The pld gene that encodes potent exotoxin has been considered as the major virulence factor for this bacterium. Deletion of pld gene is done by inserting a foreign sequence (Kanamycin gene) in between the pld sequence, thus eliminating the original sequence of the pld gene. In the future, the mutant strain produced from this study can be used for vaccine production.

Key words: Corynebacterium pseudotuberculosis, caseous lymphadenitis, pld gene

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Ramlan, M., Norlindawati, A. P., Noor Azlin Quraisah, J. & Ily Munira, M.

VETERINARY RESEARCH INSTITUTE, 59 JALAN SULTAN AZLAN SHAH, 31400 IPOH, PERAK


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