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SEQUENCE AND PHYLOGENETIC ANALYSIS OF NEWCASTLE DISEASE VIRUS GENOTYPE VII ISOLATED IN MALAYSIA DURING 1999-2012

Here we report the sequence and phylogenetic analysis of Newcastle Disease Virus (NDV) that have been circulating in Malaysia. A total of 151 NDV isolates were selected during 1999-2012 throughout Malaysia and were characterized phylogenetically. The partial region of matrix and fusion protein was amplified by reverse RT-PCR, directly sequenced and compared genetically to published sequences obtained from GenBank. The amino acid sequence of the fusion protein cleavage site revealed the presence of three different motifs; 112RRRKRF117, 112RRQKRF117 typical for velogenic strainswhile 112GKQGRL117 indicates it is from lentogenic strain. Thephylogenetic analysis revealed that 13 isolates belonged to genotype I, 2 to genotype III, 6 to genotype VI, 1 to genotype VIII and 129 to genotype VII. Isolates belonging to genotype VII were further divided into five subgenotypes; VIIa, VIIb, VIId, VIIe and VIIh. Based on the phylogenetic tree, it is found that NDV genotype VIIb and VIIe were isolated in 1999 while in year 2000 to 2009, most of the NDV isolates were NDV genotype VIId originated from China. In 2010-2012, NDV outbreaks were caused by VIIa and VIIh in Peninsular Malaysia. Interestingly, these subgenotypes have been isolated in East Malaysia since 2002. This information points to the existence of multiplegenotypes of NDV in Malaysia especially genotype VII. It is suggested that for genotype VII, no new introduction of the virus occurred during 1999-2012 but more studies should be conducted on other genes of these isolates as it may contribute to the increased virulence of the isolates.

A. S. Syamsiah , A. R. Raha , G. H. Ong, B. L. Leow, M. S. Redzuwan, M. A. Basirah, Nursyuwari N ., M. Y. Afzan and M. Ramlan

Veterinary Research Institute, Department of Veterinary Services. 59 Jalan Sultan Azlan Shah,31400 Ipoh, Perak.

Corresponding author: syamsi_aini@yahoo.com

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A. S. Syamsiah , A. R. Raha , G. H. Ong, B. L. Leow, M. S. Redzuwan, M. A. Basirah, Nursyuwari N ., M. Y. Afzan and M. Ramlan. Sequence And Phylogenetic Analysis Of Newcastle Disease Virus Genotype Vii Isolated In Malaysia During 1999-2012. VAM 2015 –Poster  & 77:25 (2015) 1 59–164 | www.jurnalteknologi.utm.my | eISSN 2180–3722 |


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