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SEQUENCE AND PHYLOGENETIC ANALYSIS OF NEWCASTLE DISEASE VIRUS ISOLATED IN MALAYSIA FROM RECENT OUTBREAKS IN 2010

Eighteen isolates of Newcastle Disease virus (NDV) were obtained from various locations in Malaysia during 2010 NDV outbreak. The isolates were phylogenetically characterized and sequenced based on partial nucleotide sequencing of the fusion (F) gene. The deduced amino acid sequences were compared with other published strains including those reported in Malaysia. All the isolates carry the same F cleavage site motif of 112R-R-R-K-R-F117 which is similar with the Malaysian NDV isolated from Sabah in 2005 (99% sequence homology). Phylogenetic analysis revealed that all the isolates were grouped in genotype VIId which is responsible for the recent outbreaks.

Keywords: Newcastle Disease Virus, poultry, Malaysia, genotype, virulent strains

Syamsiah A.S., Khairul A.M., Ong G.H., Leow B.L., Basirah M.A., M. Hasrul A.H., Zunaida B., Maizatul Z., Maizan M., And Ramlan M.

Veterinary Research Institute, Department of Veterinary Services. 59 Jalan Sultan Azlan Shah, 31400 Ipoh, Perak.

Corresponding author: syamsi_aini@yahoo.com

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Syamsiah A.S., Khairul A.M., Ong G.H., Leow B.L., Basirah M.A., M. Hasrul A.H., Zunaida B., Maizatul Z., Maizan M., And Ramlan M. Sequence and Phylogenetic Analysis of Newcastle Disease Virus Isolated In Malaysia from Recent Outbreaks In 2010. 23rd VAM Congress Proceedings (p40-41)- Oral


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