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PREVALENCE ON CASEOUS LYMPHADENITIS IN SHEEP AND GOATS DETECTED FROM CASES SUBMITTED TO VRI, IPOH FROM 2008 TO 2010

Caseous lymphadenitis (CLA) caused by Corynebacterium pseudotuberculosis in Malaysian sheep and goats were diagnosed routinely by Veterinary Research Institute. From five categories of samples received, herd health programme was analyzed because compared to other cate

SURVEILLANCE OF STRANGLES IN PENINSULAR MALAYSIA (2010)

Strangles is an extremely contagious bacterial infection specific to equine species (horses, mules and zebras). A nationwide screening of horses was carried out following an isolation of Streptococcus equi subsp. equi (S. equi) from a horse. All horses were monitored for respiratory signs, nasal

CASES OF E. COLI INFECTION SUBMITTED TO VRI IN 2009

A total of 140 cases were positive for E.coli isolation from various animal species. The highest samples isolated were organs (81%) while goat was the most common animal with E.coli infection (61%). Untypable E.coli was the common serotype isolated (91%) and highly suggestive to play a role in th

ANTIMICROBIAL DRUG RESISTANCE OF SALMONELLA TYPHIMURIUM ISOLATES FROM MEAT SAMPLES SUBMITTED TO VETERINARY RESEARCH INSTITUTE IN 2009

This study reports the antimicrobial drug resistance pattern of Salmonella Typhimurium from various meat samples submitted for diagnosis to the Veterinary Research Institute. A total of 32 Salmonella Typhimurium isolated from various meat samples from January to December 2009 were tested

COMMON LIVESTOCK DISEASES DIAGNOSED IN VETERINARY RESEARCH INSTITUTE (VRI) FROM 2006-2010

Animal diseases are diagnosed routinely in the Veterinary Research Institute.  The information obtained each year as a result of almost 400,000 samples tested for various diseases from zoonotic to exotic diseases highlights the importance of rapid and efficient diagnostic services for the co

ISOLATION OF MYCOPLASMA SPECIES FROM VARIOUS ANIMAL HOSTS IN MALAYSIA

This paper reports on the isolation of Mycoplasma sp from various animal host in Malaysia from 2006 to 2009. Samples received for Mycoplasma isolation were submitted to Avian Bacteriology, Veterinary Research Institute, Ipoh. The samples were cultured onto Pleuropneumonia Like Organism (PPLO) aga

SEQUENCE AND PHYLOGENETIC ANALYSIS OF NEWCASTLE DISEASE VIRUS ISOLATED IN MALAYSIA FROM RECENT OUTBREAKS IN 2010

Eighteen isolates of Newcastle Disease virus (NDV) were obtained from various locations in Malaysia during 2010 NDV outbreak. The isolates were phylogenetically characterized and sequenced based on partial nucleotide sequencing of the fusion (F) gene. The deduced amino acid sequences were compare

A CASE REPORT: MOLECULAR DETECTION OF MAREK’S DISEASE (MD) IN THE VETERINARY RESEARCH INSTITUTE, IPOH.

A breeder poultry farm from Perak state reported a case of emaciation and poor body condition in a poultry flock aged between week 20 to week 24. The samples received were pooled organ and carcasses. A diagnosis of Marek’s disease in the Veterinary Research Institute (VRI) was made based on

APPLICATION OF NIRS TO PREDICT CRUDE PROTEIN IN FEEDSTUFFS

The most wide spread use of near infrared spectroscopy (NIRS) has been for the determination of protein, moisture, starch, lipids and ash in feed ingredients. On-the-spot analysis of the whole sample, i.e. a non-destructive technique, allows simultaneous measurement of several parameters with hig

STRATEGIES FOR RESIDUE ANALYSIS OF VETERINARY DRUGS IN FOOD OF ANIMAL ORIGIN

The presence of residues and their associated harmful health effects on humans makes the strategies for veterinary drug residue analysis an important measure in ensuring consumer protection. These strategies include sampling and analytical approach. Emphasis is given to sample preparation and met

ANALYSIS OF CYROMAZINE IN POULTRY FEED AND MUSCLES USING LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY

Cyromazine is a registered pesticide for controlling flies  in poultry-caged layers.  The compound is added in feed and passed through the chicken, leaving a residuin the manure that controls the growth of the fly larvae developing  the area Concentration of cyromaz

DETERMINATION OF ORGANOCHLORINE PESTICIDE RESIDUES IN CHICKEN TISSUE USING QuEChERS METHOD

A rapid multiresidue procedure with gas-chromatography-mass spectrometry (GC-MS) detection has been developed to screen for thepresence of organochlorine pesticide residues in chicken tissue. The method used a new sample preparation procedure based onQuEChERS (Quick, Easy, Cheap, Effective, Rugge

MONITORING OF MELAMINE IN MILK AND FEED USING ELISA AND LCMS/MS SCREENING METHODS

A monitoring program for melamine in milk and feed was conducted in response to global melamine alertness in the year 2008. Two screening methods were adopted i.e., a liquid chromatography triple quadrupole tandem mass spectrometry (LC-MS/MS) and enzyme- linked immunosorbent assay (ELISA). The li

STUDIES ON NUTRIONALLY-RELATED BLOOD METABOLITES – TOTAL PROTEIN AND GLUCOSE LEVELS, IN GOATS OF KINTA AND HILIR PERAK DISTRICTS

The objective of this study was to examine the nutritionally-related blood  metabolites;  total  protein  and glucose levels in goats of Kinta and Hilir Perak districts. A total of eight farms were screened, involving a total of 220 animals. Serum samples were collected in pla

ANALYSIS OF QUINOLONES IN POULTRY MUSCLES USING LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY

Residues of quinolone antibiotics were determined in poultry muscle using an LC-MS/MS method coupled with electrospray ionization.  The procedure was optimised and validated for simultaneous identification, confirmation and quantitation of 12 quinolones in poultry muscle.  The antibioti
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