Marni S., Mustafa A.M. and Marzura M.R.
Residues of quinolone antibiotics were determined in poultry muscle using an LC-MS/MS method coupled with electrospray ionization. The procedure was optimised and validated for simultaneous identification, confirmation and quantitation of 12 quinolones in poultry muscle. The antibiotics included
nalidixic acid, flumequine, cinoxacin, pipemidic acid, norfloxacin, enoxacin,
ciprofloxacin, danofloxacin enrofloxacin, ofloxacin, sarafloxacin and difloxacin. The samples were extracted with glycine/HCl and purified using Oasis HLB cartridges. LC separation was achieved using Atlantis C-18 column. Multiple reactions monitoring (MRM) was used for selective detection of each quinolone while D5-norfloxacin was used as internal standard. The calibration curves were linear in the 10?300, 20-600, 30-900 and 40-1200 ?g/kg range, with typical (R2) values higher than 0.98 and 0.99. The CCa ranged between 13.94 and 433.61 ?g/kg, while CC b were ranged between 23.69 and 461.49 ?g/kg. The LOD and LOQ were much lower than the respective Maximum Residue Limits. The accuracy of the method ranged from 88 % to 119%, the coefficient of variation (CV, %) for intra-day was lower than 15%. This validated method was successfully applied to real samples for the analysis of quinolones in poultry muscles