TAN L.J., DAHLIA H., ZARRAHIMAH Z., CHANDRAWATHANI P. AND RAMLAN M.
The isolation of Mycoplasma iners from broiler chickens is reported for the first time in Malaysia. Chickens died after 2-3 days showing clinical signs of difficulties in movement. Post mortem finding showed cloudy air sac with chessy materials. The isolates were identified and characterized by biochemical tests. Confirmation of the isolates were carried out by growth inhibition test with specific antisera against M. iners (type strain PG30). The pathogenicity of the isolates was tested in chicken embryos. Results showed that M. iners caused 75% mortality in chicken embryos infected with 1 x 106 CFU/ml.