{"id":6924,"date":"2026-08-13T02:57:44","date_gmt":"2026-08-13T02:57:44","guid":{"rendered":"https:\/\/research.dvs.gov.my\/portal\/?page_id=6924"},"modified":"2026-08-13T03:21:10","modified_gmt":"2026-08-13T03:21:10","slug":"v16n2","status":"publish","type":"page","link":"https:\/\/research.dvs.gov.my\/portal\/index.php\/v16n2\/","title":{"rendered":"V16N2_pp67-80"},"content":{"rendered":"\t\t
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Malaysian Journal of Veterinary Research \u00a0|\nVol. 16, No. 2 (2025), pp. 67\u201380 \u00a0|\nPrint ISSN: 2180-3897 \u00a0 eISSN: 3122-6760<\/p>\n\n

COMPARATIVE EFFECTS OF CUMULUS CELL CO-CULTURE AND CONDITIONED MEDIUM ON IN VITRO MATURATION OF MURINE OOCYTES<\/h1>\n\n

NIK ABD RAHMAN FAHMI, L.1<\/sup>\n, REDZUAN NUL HAKIM, A.R.1,2<\/sup>*, MUHAMMAD LOKMAN,M.I.1<\/sup>, AND MUHAMMAD ALIF, M.1<\/sup><\/strong><\/p>\n\n

1<\/sup> Institute of Planetary Survival for Sustainable Well-being, International Islamic University Malaysia, 25100\nKuantan, Pahang, Malaysia
\n2<\/sup> Kulliyyah of Nursing, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
\n*<\/sup>Corresponding author: redzuan@iium.edu.my<\/p>\n\n

DOI:<\/strong>\nhttps:\/\/doi.org\/10.xxxxx\/mjvr.v6n2.2025.pp67-80<\/a><\/p>\n\n\n

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Abstract<\/h2>\n

Assisted reproductive technology plays a vital role in fertility treatment and conservation\nefforts, with in vitro maturation being a key technique in oocyte development. Cumulus cell co-culture\nand supplementation with cumulus cell co-culture secretions have been studied for their effects on in vitro\nmaturation outcomes. In this study, mice were used as the animal model to evaluate the impact of these\napproaches on oocyte morphology and grading. The results showed that oocytes matured in the cumulus\ncell co-culture system had better zona pellucida integrity (54.2%) and a higher rate of polar body formation\n(70.8%) compared to those supplemented with cumulus cell co-culture secretions, where all oocytes showed\nzona pellucida abnormalities and only 30.0% exhibited normal polar body formation (p<0.05). Oocyte grading\nrevealed a higher percentage of Grade A oocytes in the cumulus cell co-culture group (54.2%), whereas\n70.0% of oocytes in the secretion supplementation group were classified as Grade C (p<0.05). These findings\nhighlight the advantages of cumulus cell co-culture in improving oocyte quality and refining in vitro maturation\nprotocols for reproduction and conservation biology<\/p>\n\n

Keywords:<\/strong> in vitro maturation, cumulus cell co-culture, oocyte quality, assisted reproduction, conservation\nbiology<\/p>\n\n

Download Full Text (PDF)\n<\/a><\/p>\n\n\n

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How to Cite<\/h2>\n

Nik Abd Rahman Fahmi, L., Redzuan Nul Hakim, A.R., Muhammad Lokman, M.I., & Muhammad Alif, M. (2025). Comparative effects of cumulus cell co-culture and conditioned medium on in vitro maturation of murine oocytes. Malaysian Journal of Veterinary Research<\/em>, 16(2), 67-80.<\/p>\n\n

Received: XXXXXX \u00a0|\u00a0 Accepted: XXXXXX \u00a0|\u00a0 Published: XXXXXX<\/p>\n\n<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Back to Article List Malaysian Journal of Veterinary Research \u00a0| Vol. 16, No. 2 (2025), pp. 67\u201380 \u00a0| Print ISSN: 2180-3897 \u00a0 eISSN: 3122-6760 COMPARATIVE EFFECTS OF CUMULUS CELL CO-CULTURE AND CONDITIONED MEDIUM ON IN VITRO MATURATION OF MURINE OOCYTES NIK ABD RAHMAN FAHMI, L.1 , REDZUAN NUL HAKIM, A.R.1,2*, MUHAMMAD LOKMAN,M.I.1, AND MUHAMMAD ALIF, […]<\/p>\n","protected":false},"author":2,"featured_media":4789,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-6924","page","type-page","status-publish","has-post-thumbnail","hentry"],"rttpg_featured_image_url":{"full":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-scaled.jpg",2560,445,false],"landscape":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-scaled.jpg",2560,445,false],"portraits":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-scaled.jpg",2560,445,false],"thumbnail":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-150x150.jpg",150,150,true],"medium":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-300x52.jpg",300,52,true],"large":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-1024x178.jpg",640,111,true],"1536x1536":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-1536x267.jpg",1536,267,true],"2048x2048":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-2048x356.jpg",2048,356,true],"academix-single-team-thumb":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-360x270.jpg",360,270,true],"academix-team-thumbnail":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-263x216.jpg",263,216,true],"academix-event-thumbnail":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-555x370.jpg",555,370,true],"academix-event-gallery-thumb":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-227x168.jpg",227,168,true],"academix-blog-thumb":["https:\/\/research.dvs.gov.my\/portal\/wp-content\/uploads\/2021\/12\/5-01-100x80.jpg",100,80,true]},"rttpg_author":{"display_name":"Masrin Azami","author_link":"https:\/\/research.dvs.gov.my\/portal\/index.php\/author\/masrin\/"},"rttpg_comment":0,"rttpg_category":null,"rttpg_excerpt":"Back to Article List Malaysian Journal of Veterinary Research \u00a0| Vol. 16, No. 2 (2025), pp. 67\u201380 \u00a0| Print ISSN: 2180-3897 \u00a0 eISSN: 3122-6760 COMPARATIVE EFFECTS OF CUMULUS CELL CO-CULTURE AND CONDITIONED MEDIUM ON IN VITRO MATURATION OF MURINE OOCYTES NIK ABD RAHMAN FAHMI, L.1 , REDZUAN NUL HAKIM, A.R.1,2*, MUHAMMAD LOKMAN,M.I.1, AND MUHAMMAD ALIF,…","_links":{"self":[{"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/pages\/6924","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/comments?post=6924"}],"version-history":[{"count":32,"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/pages\/6924\/revisions"}],"predecessor-version":[{"id":6956,"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/pages\/6924\/revisions\/6956"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/media\/4789"}],"wp:attachment":[{"href":"https:\/\/research.dvs.gov.my\/portal\/index.php\/wp-json\/wp\/v2\/media?parent=6924"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}