Protein Content and Amino Acid Composition of Formed Edible Bird’s Nests in Malaysia

H. Wan Syahidah, M. Normah and Tony Albert

H. Wan Syahidah, M. Normah and Tony Albert

This study was conducted to determine and compare the protein content and amino
acid composition of farmed edible bird?s nest (EBN) obtained from different parts of
Malaysia. A total of 115 unprocessed EBN samples from 4 regions; East Coast
(Kelantan and Terengganu), Northern region (Perak, Kedah and Penang), Southern
region (Johor) and Sabah Sarawak were collected for this study. In this study, protein
was analysed using Kjeldahl method while amino acid analysis was performed as
described in Waters AccQ Tag Ultra Note with slight modifications using UPLC
Aquity H Class Systems with photodiode and fluorescent Aquity detector. Protein
analysis indicated that the average protein content of EBN from East Coast, Northern
region, Southern region and Sabah Sarawak were 53.6%, 53.8%, 54.3% and 52.8%
respectively. The amino acids of EBN comprised of essential amino acids and non
essential amino acids.Arginines amounting to 4.0-4.7% (major essential amino acid)
and serine amounting to 4.0-5.5% (major non essential amino acid) were the main
amino acid constituents. There was no significant difference (p>0.05) in protein
content while the amino acids content shows a significant different (p<0.05) between
essential amino acids and non essential amino acids in EBN from 4 regions.In
conclusion, this study supported other findings from researchers that EBN was high in
protein and non essential amino acid while breeding sites, climate and bird food
intake will affect the EBN nutrient composition