Determination of Organochlorine Pesticide Residues in Chicken Tissue Using QuEChERS Method

Khairunnisak M., Faridah I. and lzwan I.

A rapid multi-residue procedure with gas-chromatography-mass spectrometry (GC?MS) detection has been developed to screen for the presence of organochlorine pesticide residues in chicken tissue. The method used a new sample preparation procedure based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology. The extraction procedure consisted of a liquid extraction of the chicken tissue with acetonitrile, followed by the addition of buffering mixture, shook, centrifuged and finally purified by dispersive solid-phase extraction (d-SPE). This procedure was optimized for simultaneous screening of 20 compounds; ?-lindane, ?-lindane, d-lindane, heptachlor epoxide, g-chlordane, ?-chlordane, endosulfan (I), DDE, DDD, endosulfan (II), endrin aldehyde, endosulfansulfate, methoxychlor, endrin ketone, lindane, heptachlor, aldrin, dieldrin, endrin and DDT for routine monitoring. Six of the latter compounds have been validated for confirmatory purpose, with the calibration curves were linear in the range of 25-150 ?g/kg and r2 values higher than 0.98. The LOD and LOQ were lower than the respective Maximum Residue Limits (MRLs). The mean recoveries of the sample spiked at 100 pg/kg for lindane was 96%, heptachlor 98%, aldrin 103%, dieldrin 100%, endrin 103% and DDT 96%.